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" ... In the cases described above, this histologic procedure is invasive and quite complicated putting a financial burden, not to mention the extra strain on the patient. Liquid biopsy is an emerging and very promising technique to perform biopsies. It detects and isolates the circulating tumor DNA and exosomes as a source of genomic and proteomic information in patients with cancer. As every new technique faces technical hurdles which affect effectiveness and accuracy. New technological developments and next-generation sequencing analysis allow a broad application of liquid biopsy in a wide range of settings. Initially correlated to prognosis, liquid biopsy data are now being studied for cancer diagnosis, including screenings, and most importantly for the prediction of response or resistance to given treatments. In particular, the identification of specific mutations in target genes can be detected more easily as liquid biopsies are easier to be taken. That translates to more data for the physicians to analyze in real time with a specific treatment and decide on the appropriateness of treatment or the identification of secondary resistance, aiming to diagnose disease progression early enough. ... "